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cd4 cd8 til microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd4 cd8 til microbeads
    Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg <t>CD4</t> T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted <t>CD8</t> T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.
    Cd4 Cd8 Til Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+cd8+til+microbeads/CD4%2FCD8+(TIL)+MicroBeads%2C+mouse/pmc12824915-419-5-8
    Average 95 stars, based on 32 article reviews
    cd4 cd8 til microbeads - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination"

    Article Title: Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination

    Journal: iScience

    doi: 10.1016/j.isci.2025.114572

    Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg CD4 T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted CD8 T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.
    Figure Legend Snippet: Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg CD4 T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted CD8 T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.

    Techniques Used: Injection, Flow Cytometry

    Related Articles

    other:

    Article Title: Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination
    Article Snippet: CD4/CD8 (TIL) MicroBeads, mouse , Miltenyi Biotec , Cat #: 130-116-480.

    Article Title: An immunobiliary single-cell atlas resolves crosstalk between type 2 conventional dendritic cells and γδ T cells in cholangitis.
    Article Snippet: An anti-rabbit secondary antibody conjugated to AP was applied (Polyview Plus AP reagent, ENZO Life Sciences GmbH, Lörrach, Germany) and the signal was visualized using Permanent AP Red (Zytovision GmbH, Bremerhaven, Germany).

    Isolation:

    Article Title: Oncolytic Adenovirus Armed with cGAS Activates STING Pathway and Enhances Antitumor Immunity in Lung Cancer with Superior Combined Efficacy of PD-L1 Therapy
    Article Snippet: Samples were acquired on a FACSCanto II system or FACSFortessa system (BD Biosciences, CA, USA), and data were analyzed using FlowJo software (BD Biosciences, CA, USA). .. CD4 + and CD8 + TILs were isolated from both treated and untreated tumor tissues using CD4/CD8 (TIL) MicroBeads (Miltenyi, 130-116-480, German). ..

    Article Title: HTGTS-TCR-Seq for Profiling of Mouse and Human T-Cell Receptor α and β Gene Rearrangements and Diversity.
    Article Snippet: .. Flow Cytometry and Cell Sorting: For isolation of DN3 T cells from youngmice, CD4+ and CD8+ thymocytes were depleted by CD4/CD8 (TIL) MicroBeads (130-116-480, Miltenyi). .. The flow-through was subsequently stained with PE-anti-CD25 (PC61, Biolegend, 102007), FITC-anti-CD44 (IM7, Biolegend, 103006), and Fixable Viability Stain 510 (BD Pharmingen, 564406).

    Article Title: MAP4K4 Regulates the Immune Landscape of Pancreatic Tumor Microenvironment and Provides an Opportunity for Immunotherapy.
    Article Snippet: Due to its widespread resistance to conventional therapy, Pancreatic Ductal Adenocarcinoma (PDAC) is expected to be the second most common cause of cancer-related death in the United States by 2030.. One of the major impediments to therapeutic failure in PDAC is the presence of an immunosuppressive tumor microenvironment (TME).. Here, we report the effect of MAP4K4 in regulating innate and adaptive immunity in PDAC-TME in KPC tumors.

    Article Title: HTGTS‐TCR‐Seq for Profiling of Mouse and Human T‐Cell Receptor α and β Gene Rearrangements and Diversity
    Article Snippet: .. For isolation of DN3 T cells from young mice, CD4 + and CD8 + thymocytes were depleted by CD4/CD8 (TIL) MicroBeads (130‐116‐480, Miltenyi). .. The flow‐through was subsequently stained with PE‐anti‐CD25 (PC61, Biolegend, 102007), FITC‐anti‐CD44 (IM7, Biolegend, 103006), and Fixable Viability Stain 510 (BD Pharmingen, 564406).

    Flow Cytometry:

    Article Title: HTGTS-TCR-Seq for Profiling of Mouse and Human T-Cell Receptor α and β Gene Rearrangements and Diversity.
    Article Snippet: .. Flow Cytometry and Cell Sorting: For isolation of DN3 T cells from youngmice, CD4+ and CD8+ thymocytes were depleted by CD4/CD8 (TIL) MicroBeads (130-116-480, Miltenyi). .. The flow-through was subsequently stained with PE-anti-CD25 (PC61, Biolegend, 102007), FITC-anti-CD44 (IM7, Biolegend, 103006), and Fixable Viability Stain 510 (BD Pharmingen, 564406).

    FACS:

    Article Title: HTGTS-TCR-Seq for Profiling of Mouse and Human T-Cell Receptor α and β Gene Rearrangements and Diversity.
    Article Snippet: .. Flow Cytometry and Cell Sorting: For isolation of DN3 T cells from youngmice, CD4+ and CD8+ thymocytes were depleted by CD4/CD8 (TIL) MicroBeads (130-116-480, Miltenyi). .. The flow-through was subsequently stained with PE-anti-CD25 (PC61, Biolegend, 102007), FITC-anti-CD44 (IM7, Biolegend, 103006), and Fixable Viability Stain 510 (BD Pharmingen, 564406).

    Cell Isolation:

    Article Title: MAP4K4 Regulates the Immune Landscape of Pancreatic Tumor Microenvironment and Provides an Opportunity for Immunotherapy.
    Article Snippet: Due to its widespread resistance to conventional therapy, Pancreatic Ductal Adenocarcinoma (PDAC) is expected to be the second most common cause of cancer-related death in the United States by 2030.. One of the major impediments to therapeutic failure in PDAC is the presence of an immunosuppressive tumor microenvironment (TME).. Here, we report the effect of MAP4K4 in regulating innate and adaptive immunity in PDAC-TME in KPC tumors.



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    Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg <t>CD4</t> T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted <t>CD8</t> T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.
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    Image Search Results


    Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg CD4 T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted CD8 T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.

    Journal: iScience

    Article Title: Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination

    doi: 10.1016/j.isci.2025.114572

    Figure Lengend Snippet: Tregs traffic from the tumor to the TdLN at higher proportions and are equally susceptible to radiation as non-Treg CD4 T cells (A) Kaede mice were injected with either MC38, MOC1, or MOC2 cancer cells subcutaneously. Tumors were photoconverted on day 14 via ultraviolet light. TdLNs were harvested on day 3 post-photoconversion. All cell populations were pre-gated on live singlets. (B) Photoconverted CD8 T cells were defined as CD3 + CD4 − CD8 + Red + . Photoconverted Tregs were defined as CD3 + CD8 − CD4 + CD25 + Red + . Photoconverted non-Treg CD4 T cells were defined as CD3 + CD8 − CD4 + CD25 − Red + . (C) Frequencies of photoconverted CD8 T cells, Tregs, and non-Treg CD4 T cells were quantified via flow cytometry. (D) Mice were injected with MC38 cancer cells subcutaneously. Tumors were treated with 12 Gy RT on day 14 and harvested for analysis via flow cytometry on day 1–3 post-RT. Non-Treg CD4 T cells and Tregs frequencies as a percentage of live cells were quantified. (E) MC38-bearing Kaede mouse tumors were photoconverted on day 14 followed by immediate treatment with 12 Gy RT. (F) TdLNs were harvested on day 1–3 and percentages of photoconverted non-Treg CD4 T cells and Tregs were analyzed via flow cytometry. A total of 5–6 mice were analyzed per group. Data are represented as mean ± SD. Statistics were performed using an unpaired Student’s t test between groups. ns = not significant, ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.

    Article Snippet: Samples were enriched magnetically using CD4/CD8 TIL MicroBeads (Miltenyi Biotec, 130-116-480).

    Techniques: Injection, Flow Cytometry